To prepare a 5% w/v (weight/volume) sucrose solution, you can follow the following procedure:
Weigh the required amount of sucrose: Determine the mass of sucrose needed to make the solution. Since you want a 5% w/v solution, it means 5 grams of sucrose per 100 mL of solution. So, if you want to prepare a specific volume, adjust the amount accordingly. For example, for a 500 mL solution, you would need (5 g/100 mL) × 500 mL = 25 grams of sucrose.
Measure the required volume of solvent: In this case, the solvent will be water. Measure the appropriate volume of water to dissolve the calculated amount of sucrose. For example, if you want to prepare a 500 mL solution, you would measure 500 mL of water.
Dissolve the sucrose: Take a clean container and add the measured volume of water. Gradually add the calculated amount of sucrose to the water while stirring continuously. Keep stirring until all the sucrose has dissolved.
Adjust the final volume: If the solution does not reach the desired final volume after dissolving the sucrose, add more water to achieve the desired volume. Mix well to ensure homogeneity.
Optional: Filter the solution: If there are any undissolved particles or impurities, you can filter the solution using a filter paper or a suitable filtration apparatus to obtain a clear solution.
Remember to label the container properly with the concentration, date of preparation, and any other relevant information.
Please note that this procedure assumes the use of pure sucrose and water. If you are using different concentrations or other solvents, the procedure may vary.