Question

1. If I dilute 100 microliter of a solution to a tube sample containing 50mL DI Water, what's my dilution factor?

Answer 1

To calculate the dilution factor, you need to divide the final volume of the diluted solution by the initial volume of the solution added.

In this case, the initial volume of the solution added is 100 microliters (μL), and the final volume of the diluted solution is 50 mL.

First, convert the final volume from milliliters (mL) to microliters (μL):

50 mL = 50,000 μL

Now, divide the final volume by the initial volume to determine the dilution factor:

Dilution factor = Final volume / Initial volume Dilution factor = 50,000 μL / 100 μL Dilution factor = 500

Therefore, the dilution factor is 500.