To make a 1:100 dilution in 50 mL, you will need to mix 0.5 mL of the original solution with 49.5 mL of the diluent (usually a solvent like water or buffer). Here's how you can do it:
Take a clean container, such as a beaker or a graduated cylinder, capable of holding at least 50 mL.
Add 0.5 mL of the original solution to the container. You can use a pipette or a syringe for precise measurements.
Add 49.5 mL of the diluent (solvent) to the container. Again, you can use a pipette, a graduated cylinder, or any other suitable measuring tool.
Mix the contents thoroughly to ensure a homogenous dilution. You can gently swirl the container or use a stirrer if necessary.
After following these steps, you will have a 1:100 dilution of the original solution in a total volume of 50 mL.